摘要：目的 研究甘肅8個(gè)主產(chǎn)縣紅芪藥材的傅里葉變換紅外光譜（FT-IR）指紋特征，為不同產(chǎn)地紅芪的鑒別應(yīng)用提供依據(jù)。方法 采用FT-IR技術(shù)，采集甘肅省8個(gè)紅芪主產(chǎn)縣110批紅芪樣品在4000～400 cm-1范圍內(nèi)的指紋圖譜數(shù)據(jù)，對(duì)指紋圖譜的共有模式進(jìn)行光譜解析，對(duì)8個(gè)主產(chǎn)縣紅芪的FT-IR指紋圖譜進(jìn)行相似度分析，比較不同主產(chǎn)縣紅芪的FT-IR指紋特征。結(jié)果 8個(gè)主產(chǎn)縣紅芪FT-IR指紋圖譜的平均相似度大小排序?yàn)殄床?禮縣>西和>武都>漳縣>岷縣>隴西>渭源，隴西、渭源紅芪與其他產(chǎn)地的樣品差異較大；隴南地區(qū)（宕昌、禮縣、西和、武都）紅芪的FT-IR指紋圖譜較為相似，平均相似度較大，而定西地區(qū)（漳縣、岷縣、隴西、渭源）紅芪的FT-IR指紋圖譜較為相似，平均相似度較小；每個(gè)主產(chǎn)縣紅芪的FT-IR指紋圖譜均具有顯著、唯一的FT-IR指紋特征。結(jié)論 根據(jù)FT-IR指紋特征能夠?qū)崿F(xiàn)甘肅8個(gè)主產(chǎn)縣紅芪藥材的鑒別應(yīng)用。
　　關(guān)鍵詞：紅芪；傅里葉變換紅外光譜；指紋圖譜；相似度分析；指紋鑒別
　　DOI：10.3969/j.issn.1005-5304.2018.11.014
　　中圖分類號(hào)：R284.1 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：1005-5304（2018）11-0062-06
　　Abstract： Objective To study the FT-IR fingerprint characteristics of Hedysari Radix from 8 producing counties in Gansu Province； To provide references for identification and application of Hedysari Radix in different producing counties. Methods FT-IR fingerprints of 110 batches of Hedysari Radix from 8 producing counties in Gansu Province were collected in the wave number range of 4000–400 cm-1. The common pattern of the fingerprints were analyzed， and the similarity analysis were used to analyze the FT-IR fingerprints of Hedysari Radix from 8 producing counties. The FT-IR fingerprint characteristics of Hedysari Radix from 8 producing counties in Gansu Province were compared. Results The rank of average similarity of FT-IR fingerprints of Hedysari Radix from 8 producing counties was Tanchang County > Li County > Xihe County > Wudu District > Zhang County > Min County > Longxi County > Weiyuan County， and Hedysari Radix from Longxi County and Weiyuan County were very different from other producing counties. The FT-IR fingerprints of Hedysari Radix from Longnan City （Tanchang County， Li County， Xihe County and Wudu District） were similar， and the average similarity was relatively high； while that from Dingxi City （Zhang County， Min County， Longxi County and Weiyuan County） were similar， and the average similarity was relatively low. Hedysari Radix from every producing county had a significant and unique FT-IR fingerprint characteristic. Conclusion The identification and application of Hedysari Radix from 8 producing counties in Gansu Province can be realized according to FT-IR fingerprint characteristics.
　　Keywords： Hedysari Radix； FT-IR； fingerprint； similarity analysis； fingerprint identification
　　紅芪是豆科巖黃芪屬植物多序巖黃芪Hedysarum polybotrys Hand.-Mazz的干燥根，最早記載于《神農(nóng)本草經(jīng)》黃芪項(xiàng)下，被列為上品，入藥已有2000多年歷史[1]。紅芪有補(bǔ)氣固表、利尿、托毒排膿、斂瘡生肌等功效[2]，在免疫調(diào)節(jié)、抗腫瘤、抗突變及抗損傷等方面作用突出[3]。
　　紅芪是甘肅特有的道地藥材，主產(chǎn)于甘肅隴南地區(qū)的武都、禮縣、宕昌及定西地區(qū)的岷縣、隴西、漳縣等地，產(chǎn)量占全國(guó)95%以上。其中，隴南是甘肅省唯一屬于長(zhǎng)江水系并擁有亞熱帶氣候的地區(qū)，而定西則屬于中溫帶半干旱區(qū)。研究發(fā)現(xiàn)，不同時(shí)間、空間的氣候條件、水土異質(zhì)等環(huán)境變化均會(huì)影響藥用植物的物候規(guī)律、生長(zhǎng)發(fā)育、能量代謝、物質(zhì)合成及氣體交換等生理作用，繼而影響其次生代謝產(chǎn)物的變化，從而導(dǎo)致藥材質(zhì)量及臨床療效有所差異[4]。因此，探討不同環(huán)境因子背景下紅芪藥材的物質(zhì)基礎(chǔ)、遺傳效應(yīng)、藥理作用等差異，對(duì)于突出紅芪的產(chǎn)地比較優(yōu)勢(shì)、打造地域性紅芪品牌具有重要的實(shí)際意義。本研究對(duì)甘肅8個(gè)主產(chǎn)縣紅芪藥材的傅里葉變換紅外光譜（FT-IR）指紋圖譜進(jìn)行相似性分析及差異比較，旨在發(fā)現(xiàn)甘肅不同主產(chǎn)縣紅芪的FT-IR指紋特征，為不同產(chǎn)地紅芪的鑒別應(yīng)用提供依據(jù)。

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