幾種中藥材對(duì)兔原代腎小管上皮細(xì)胞增殖作用影響的研究
發(fā)布時(shí)間:2019-08-29 來源: 日記大全 點(diǎn)擊:
摘要:通過體外培養(yǎng)兔原代腎小管上皮細(xì)胞,來研究大血藤、吳茱萸、豨薟草及川牛膝4種中藥材提取物對(duì)腎小管上皮細(xì)胞增殖作用的影響。利用MTT法測定大血藤、吳茱萸、豨薟草及川牛膝4種中藥材的水提與醇提的3mg/L,30mg/L和300mg/L三個(gè)濃度組對(duì)兔原代腎小管上皮細(xì)胞增殖作用的影響。結(jié)果表明,吳茱萸和豨薟草的醇提300mg/L濃度組及大血藤的水提300mg/L濃度組對(duì)兔原代腎小管上皮細(xì)胞的增殖作用抑制很強(qiáng),其存活率分別僅有24.12%,16.03%和30.84%,而大血藤的醇提3mg/L濃度組和豨薟草的醇提30mg/L及3mg/L濃度組對(duì)兔原代腎小管上皮細(xì)胞的增殖作用具有促進(jìn)作用,其存活率達(dá)到了107.91%,114.95%和111.45%。大血藤、吳茱萸、豨薟草及川牛膝4種中藥材對(duì)兔原代腎小管上皮細(xì)胞增殖作用均有不同程度的影響。
關(guān)鍵詞:大血藤;吳茱萸;豨薟草;川牛膝;增殖作用
中圖分類號(hào):R914
文獻(xiàn)標(biāo)識(shí)碼:A
文章編號(hào):1008-0457(2017)04-0076-04 國際DOI編碼:10.15958/j.cnki.sdnyswxb.2017.04.014
Abstract:The effects of extracts of four Chinese herbal medicines, namely Sargentodoxa cuneata、Tetradium ruticarpum、Siegesbeckia orientalisL and Cyathula officinalis Kuan, on the proliferation of renal tubular epithelial cells were studied by culturing rabbit primary renal tubular epithelial cells in vitro. The effects of 3mg/L, 30 mg / L and 300 mg / L of water extraction and alcohol extraction of the four herbal medicines on tubule epithelial cell proliferation were studied by MTT method. The results showed that 300 mg / L of the alcohol extract of T. ruticarpum and S. orientalis and the water extract of 300 mg / L of S. cuneata significantly inhibited the proliferation of primary renal tubular epithelial cells by 24.04%, 16.03% and 30.84%, respectively. 3 mg / L of the alcohol extract of S. cuneata and 30 mg / L and 3 mg / L of alcohol extract of S. orientalis showed a proliferation effect on primary renal tubular epithelial cells, and the survival rate reached 107.91%, 114.95% and 111.45%. All of the four Chinese herbal medicines had some effects on the proliferation of primary renal tubular epithelial cells in rabbit.
Key words:sargentodoxa cuneata; tetradium ruticarpum; siegesbeckia orientalisL; cyathula officinalis kuan; proliferation effect
大血藤(Sargentodoxa cuneata),木通科大血藤屬植物,具有抗炎,活血,祛風(fēng)止痛等藥理作用。吳茱萸(Tetradium ruticarpum),蕓香科吳茱萸屬植物,具有止瀉、散寒止痛等藥理作用。豨簽草(Siegesbeckia orientalis L.)為菊科草本植物,具有祛風(fēng)濕,利關(guān)節(jié),抗炎等藥理作用。川牛膝(Cyathula officinalis Kuan),莧科杯莧屬植物,具有逐瘀通經(jīng),通利關(guān)節(jié),降血壓作用等藥理作用[1]。這4種中藥材均具有溫補(bǔ)腎陽、利水滲濕、益氣活血等功效,根據(jù)中醫(yī)藥理論中的性味歸經(jīng)之說,均可歸屬為腎經(jīng)。根據(jù)歸經(jīng)理論,能夠?qū)⑺幬锏淖饔门c人體的臟腑經(jīng)脈之間的關(guān)系相結(jié)合,說明藥物能治療或作用的臟腑經(jīng)脈的病變,從而就能具體的認(rèn)識(shí)到藥物的主要作用器官和藥物的特殊功效[2]。本文旨在利用原代分離的兔腎小管上皮細(xì)胞對(duì)大血藤、吳茱萸、豨薟草及川牛膝4種中藥材提取物對(duì)腎小管上皮細(xì)胞增殖作用的影響進(jìn)行研究,為這4種中藥材的應(yīng)用及腎毒性作用的研究提供參考。
1 材料與方法
1.1 材料
20天齡雄兔購自貴州省畜牧研究所實(shí)驗(yàn)基地,清潔級(jí),合格證CSXK(黔)2012-001。
中藥材:大血藤、吳茱萸(四平市百草堂中藥飲片有限公司);豨薟草(四川省川南藥業(yè)有限公司);川牛膝(成都市益誠藥業(yè)有限公司)。
試劑:DMEM/F12(1∶1)(Hyclone),胎牛血清(四季青),胰蛋白酶(Amresco),堿性磷酸酶染液—鈣鈷法(南京建成),SP染液、CK18(北京博奧森),DAB顯色劑(北京中杉金橋),噻唑藍(lán)(MTT),二甲亞砜(DMSO)。
熱點(diǎn)文章閱讀